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مجله پژوهش در بهداشت محیط

مجله پژوهش در بهداشت محیط

بررسی اثرات ضدمیکروبی عصاره‌ی اتانولی گیاه برزا (Johernia aromatica) بر میکروارگانیسم‌های استافیلوکوکوس‌اورئوس، اشرشیاکلی، کلستریدیوم‌پرفرینجنس و مخمر کلویورومایسس‌مارکسیانوس

نوع مقاله : مقالات پژوهشی

نویسندگان
آرام سلیمی
فردین میراحمدی
صنایع غذایی، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد سنندج، سنندج، ایران.
10.22038/jreh.2024.25293
چکیده
زمینه و هدف: استفاده از خواص ضدمیکروبی گیاهان دارویی می تواند اثرات ضدمیکروبی قابل‌ملاحظه ای داشته  باشد. از این‌رو پژوهش کنونی با هدف بررسی اثرات ضدمیکروبی عصاره‌ی اتانولی گیاه برزا بر میکروارگانیسم های استافیلوکوکوس  اورئوس (گرم مثبت)، اشرشیاکلی (گرم منفی)، کلستریدیوم پرفرینجنس و مخمر کلویورومایسس مارکسیانوس انجام شد. 

مواد و روش‌ها : اثر ضدمیکروبی عصاره ی الکلی گیاه برزا به روش دیسک دیفیوژن، چاهک آگار، حداقل غلظت مهارکنندگی و حداقل غلظت کشندگی در میکروارگانیسم های مطالعه مورد بررسی قرار گرفت. هم چنین ترکیبات موجود در عصاره ی اتانولی گیاه برزا توسط دستگاه (GC-Mass) تعیین شد. تجزیه وتحلیل داده ها با استفاده از نرم‌افزار SPSS نسخه ی 25 در سطح 5 درصد انجام پذیرفت. 

یافته‌ها: براساس نتایج، کلستریدیوم پرفرینجنس و  استافیلوکوکوس  اورئوس حساس ترین میکروارگانیسم ها نسبت‌ به رقت‌های 18/5، 37، 75 و 150 بودند. حداقل میزان مهارکنندگی عصاره ی مورد مطالعه برای باکتری گرم مثبت کلستردیوم‌پرفریجنس در حدود 8 میلی گرم ‌بر ‌میلی لیتر بود. هم چنین نتایج نشان داد که حداقل غلظت کشندگی برای تمام سویه های مورد استفاده در این پژوهش به جز سویه ی مخمری کلویورومایسس مارکسیانوس برابر با 1024 میلی‌گرم بر میلی لیتر به دست  آمد.

نتیجه‌گیرى: عصاره ی اتانولی گیاه برزا در محیط آزمایشگاهی فعالیت ضد‌‌‌میکروبی قابل‌توجهی روی سویه‌های مورد‌ مطالعه نشان داد. بنابراین لازم است که مطالعات بیش‌تری در مورد این گیاه انجام گیرد تا بتوان آن را به‌عنوان یک ماده ی ضد‌میکروبی طبیعی و جدید معرفی نمود.
کلیدواژه‌ها
عصاره اتانولی
اثرات ضدباکتریایی
گیاه برزا
گیاهان دارویی

عنوان مقاله English

Crobial Effects of the Ethanolic Extract of Barza Plant (Johernia Aromatica (on the Microorganisms Staphylococcus Aureus, Escherichia Coli, Clostridium Perfringens and Kluyoromyces Marcianos Yeast

نویسندگان English

Aram Salimi
Fardin Mir Ahmadi
Food Industry, Faculty of Agriculture, Islamic Azad University, Sanandaj Branch, Sanandaj, Iran.
چکیده English

Background and Purpose: Using the antimicrobial properties of medicinal plants can have significant antimicrobial effects. Therefore, the present study was conducted with the aim of investigating the antimicrobial effects of the ethanolic extract of the plant on the microorganisms Staphylococcus aureus (gram positive), Escherichia coli (gram negative), Clostridium perfringens, and the yeast Chloivromyces marcianus.

Materials & Methods: Antimicrobial effect of alcoholic extract of Barza plant was investigated by disk diffusion method, agar well, minimum inhibitory concentration and minimum lethal concentration in the studied microorganisms. Also, the compounds in the ethanolic extract of Barza plant were determined by GC-Mass. Data analysis was done using SPSS version 25 software at the 5% level. 

Results: According to the results, Clostridium perfringens and Staphylococcus aureus were the most sensitive microorganisms to 18.5, 37, 75 and 150 dilutions. The minimum inhibitory level of the studied extract for the gram-positive bacterium Clostridium perfringens was around 8 mg/ml. Also, the results showed that the minimum lethality concentration for all the strains used in this research, except for the yeast strain Chloromyces Marcianos, was equal to 1024 mg/ml.

Conclusion: The ethanol extract of the plant in the laboratory environment showed considerable anti -immigration activity on the seats. Therefore, further studies need to be conducted on this plant to be introduced as a natural and natural anticoagulant.
 
Open Access Policy: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/

کلیدواژه‌ها English

Ethanolic Extract
Antibacterial Effects
Johernia Aromatica
Herbal Medicine
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