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مجله پژوهش در بهداشت محیط

مجله پژوهش در بهداشت محیط

بررسی آلودگی میکروبی وسایل و تجهیزات در آزمایشگاه‌های آموزشی دانشگاه علوم پزشکی کاشان در سال 1397

نوع مقاله : Research Paper

نویسندگان
علی نظری عالم 1
حسن رحمانی 2
حسین اکبری 3
محمد علی اسدی 4
مائده قوامی 5
مریم بهاری 5
ناهید یسلیانی 5
1 دانشیار گروه میکروب شناسی و ایمنولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی کاشان، کاشان، ایران
2 استادیار گروه مهندسی بهداشت محیط، دانشگاه علوم پزشکی کاشان، کاشان، ایران.
3 دانشیار گروه آمار زیستی و اپیدمیولوژی، دانشکده بهداشت، دانشگاه علوم پزشکی کاشان، کاشان، ایران
4 مربی گروه انگل‏شناسی و قارچ‏شناسی، دانشگاه علوم پزشکی کاشان، کاشان، ایران
5 کارشناسی مهندسی بهداشت محیط، دانشکده بهداشت، دانشگاه علوم پزشکی کاشان، کاشان، ایران
10.22038/jreh.2024.24892
چکیده
زمینه و هدف: آزمایشگاه‌ها بدلیل بالا بودن تعداد پرسنل و دانشجویان، تنوع نمونه‌ها و آلودگی نمونه‌ها به عوامل بیماری زای مختلف از محیط‌های حساس و مخاطره آمیز برای انتقال و انتشار بسیاری از بیماری‌ها می‌باشند. لذا هدف مطالعه‌ی حاضر، بررسی آلودگی باکتریایی و قارچی تجهیزات آزمایشگاه‌های آموزشی دانشگاه علوم پزشکی کاشان بود.

مواد و روش‌ها: این مطالعه توصیفی مقطعی که درسال 1397 در دانشگاه علوم پزشکی انجام گردید و به‌طور تصادفی نمونه‌گیری از 155 دستگاه توسط یک نفر نمونه‌گیر ثابت انجام گردید. پس از جمع‌آوری اطلاعات، فراوانی آلودگی میکروبی و قارچی برای هر آزمایشگاه و هر وسیله محاسبه شده و جداول فراوانی ترسیم و همچنین جداول فراوانی برحسب نوع باکتری یا قارچ و کلنی‌های رشدکرده محاسبه گردید.

یافته هـا: از 155 مورد نمونه‌گیری و کشت انجام‌شده از تجهیزات آزمایشگاه‌های دانشکده‌های دانشگاه علوم پزشکی کاشان در مجموع بیشترین درصد آلودگی (83/22% ( مربوط به گونه‌ی باسیلوس (129 مورد) و 17مورد (10/96% (گونه‌ی سودوموناس و 17 مورد (10/96%) مربوط به گونه‌ی اشرشیاکلی بود. از مجموع تمام نمونه‌های گرفته‌شده فقط 7 نمونه فاقد آلودگی بود و اکثر تجهیزات آلوده بودند. 

نتیجه گیری: یافتـه‌ها نـشان داد اکثر تجهیزات آزمایشگاهی آموزشی دانشگاه علوم پزشکی کاشان دارای آلـودگی میکروبـی بـا باکتری‌هـای گـرم مثبـت و منفـی و عوامـل قـارچی می‌باشد. 
کلیدواژه‌ها
آلودگی میکروبی
آلودگی قارچی
تجهیزات آزمایشگاهی
دانشگاه علوم پزشکی کاشان

عنوان مقاله English

Microbial Contamination of Tools and Equipment Used in Educational Laboratories of Kashan University of Medical Sciences in 2018

نویسندگان English

Ali Nazari-Alam 1
Hasan Rahmani 2
Hossein Akbari 3
Mohammad Ali Asadi 4
Maedeh Qavami 5
Maryam Bahari 5
Nahid Yesliani 5
1 Associate Professor, Department of Microbiology, Kashan University of Medical Sciences, Kashan, Iran; Email: nazarialam.ali@gmail.com.
2 Assistant Professor, Department of Environmental Health Engineering, Kashan University of Medical Sciences, Kashan, Iran.
3 Associate Professor, Department of Epidemiology and Biostatistics, School of Health Kashan University of Medical Sciences, Kashan, Iran
4 Instructor of the Department of Parasitology and Mycology, Kashan University of Medical Sciences, Kashan, Iran
5 Bachelor of Environmental Health Engineering, Faculty of Health, Kashan University of Medical Sciences, Kashan, Iran
چکیده English

Background and Objective: Laboratories, due to their large workforce, diverse sample types, and contamination by various pathogenic agents, are considered high-risk environments for the transmission and spread of numerous diseases. This study aimed to investigate bacterial and fungal contamination of laboratory equipment in the educational laboratories at Kashan University of Medical Sciences.

Materials and Methods: This cross-sectional descriptive study was conducted in 2018 at Kashan University of Medical Sciences. A single consistent sampler performed a random sampling of 155 devices. After data collection, the frequency of microbial and fungal contamination was calculated for each laboratory and each piece of equipment. Frequency tables were constructed based on the type of bacteria or fungi and the colonies grown.

Results: Among the 155 samples collected and cultured from laboratory equipment across various faculties, the highest percentage of contamination (83.22%) was observed with the Bacillus species (129 cases), followed by Pseudomonas species (17 cases, 10.96%) and Escherichia coli (17 cases, 10.96%). Only 7 samples were free of contamination, indicating widespread contamination across most equipment.

Conclusion: The results indicate that the majority of educational laboratory equipment at Kashan University of agents Medical Sciences is contaminated with both Gram-positive and Gram-negative bacteria, as well as fungal.
 
 
Open Access Policy: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/

کلیدواژه‌ها English

Microbial Contamination
Fungal Contamination
Laboratory Equipment
Kashan University of Medical Sciences
  1. Shojaee, S., et al., The Rate of Occupational Exposure to Patients' Specimen among Personnel of Medical Diagnostic Laboratories in Birjand City. Medical Laboratory Journal, 2013; 7(2): 30-36. http://mlj.goums.ac.ir/article-1-273-en.html
  2. Jokar, A., et al., A Comparison of Efficacy of Isopropyl Alcohol and Ethanol in Disinfection Programs in Pediatrics Ward and Neonatal Intensive Care Unit. Hayat, 2009; 15 (3) :52-58. http://hayat.tums.ac.ir/article-1-113-en.html
  3. Valian, A., et al., Evaluation of the Aerobic Bacterial Contamination of Students Gowns in Restorative and Periodontics Departments of Dental School of Shahid Beheshti University of Medical Sciences in 2013. Journal of Mashhad Dental School, 2015; 39(2): 181-190. https://doi.org/10.22038/jmds.2015.4436
  4. Rabieyan, M., et al., Evaluation of accidental exposures in laboratories' personnel in Teaching Hospitals of Tehran University of Medical Sciences(TUMS) during 2004 to 2005. Payavard Salamat, 2008; 2(3): 33-41. http://payavard.tums.ac.ir/article-1-140-en.html
  5. Stanley, S.K., Guidelines for the use of antiretroviral agents in HIV-infected adults and adolescents. Morbidity and Mortality Weekly Report: Recommendations and Reports, 1998; 39-82. https://doi.org/10.7326/0003-4819-137-5_part_2-200209031-00001
  6. Maddi Neshat, M. and F. Pashayee, Experience of health care workers after contact with sharp instruments contaminated with patients 'blood: A phenomenological -qualitative study . J Journal of North Khorasan University of Medical Sciences. Journal of North Khorasan University if Medical Sciences, 2011; 3(3): p. 83-90. https://doi.org/10.29252/jnkums.3.3.83
  7. Rashidi, A., et al., Microbial contamination and antibiotic resistance of bacterial isolates from disposable food containers in Hamadan. Pajouhan scientific journal, 2015; 13(4): 27-33. http://psj.umsha.ac.ir/article-1-149-en.html
  8. Kallner, A., et al., The Stockholm Consensus Conference on quality specifications in laboratory medicine, 25-26 April 1999. 1999; 59(7): 475-475. https://doi.org/10.1080/00365519950185175 PMid:10667681
  9. Sheila Jalalpour, et al., Surveying the Frequency of β-lactamase Enzyme and Antibiotic Sensitivity Pattern in Isolated Pathogen Bacteria from Low and High Hospital Contact Surfaces %J Pajoohande. 2010; 15(2): 77-82. http://pajoohande.sbmu.ac.ir/article-1-910-en.html
  10. Peleg, A.Y. and D.C. Hooper, Hospital-acquired infections due to gram-negative bacteria. New England Journal of Medicine, 2010; 362(19): 1804-1813. https://doi.org/10.1056/NEJMra0904124
  11. Ayatollahi, A.A., et al., Assessing the prevalence of Gram Negative Bacilli Isolated from Hospitals' Equipment and Surfaces in Western Region of Golestan province. Hospital, 2016; 15(2): 67-73. https://doi.org/10.1556/1886.2017.00015
  12. Dancer, S.J., Controlling hospital-acquired infection: focus on the role of the environment and new technologies for decontamination. Clinical microbiology reviews, 2014; 27(4): 665-690. https://doi.org/10.1128/CMR.00020-14
  13. mohsen, t., et al., Effect of ethanol on bacterial contamination of computer keyboards in two Semnan medical centers. Iranian Journal of Infectious Diseases and Tropical Medicine, 2017; 21(75): 15-9.
  14. Pasanen, A.-L., et al., Occurrence and moisture requirements of microbial growth in building materials. International Biodeterioration & Biodegradation, 1992; 30(4): 273-283. https://doi.org/10.1016/0964-8305(92)90033-K
  15. Hoshyar, F., et al., Study of fungal infection frequency in libraries affiliated with Shahrekord University of Medical Sciences in 2013. Journal of Shahrekord Uuniversity of Medical Sciences, 2014; 16(4): 39-45. https://doi.org/10.34172/jsums.2019.17
  16. Seçgin, Y., et al., Analysis of an anatomy laboratory for microbiological contamination. Northwestern Medical Journal, 2024; 4(2): 106-112. https://doi.org/10.54307/2024.NWMJ.114
  17. Manshad, D.R., et al. Isolation of bacterial and fungal contaminants from classrooms at Samawah Technical Institute. in AIP Conference Proceedings. 2024. AIP Publishing. https://doi.org/10.1063/5.0191702
  18. Gund, M.P., et al., Bacterial contamination potential of personal protective equipment itself in dental aerosol-producing treatments. Odontology, 2024; 112(2): 309-316. https://doi.org/10.1007/s10266-023-00848-3
  19. Firoozeh, F., et al., Microbial contamination of pumice used in dental laboratories. Healthcare in Low-resource Settings, 2013. 1(1): e5-e5. https://doi.org/10.4081/hls.2013.e5
  20. Selim, H.S. and A.F. Abaza, Microbial contamination of mobile phones in a health care setting in Alexandria, Egypt. GMS hygiene and infection control, 2015; 10. https://doi.org/10.3205/dgkh000246
  21. Muhadi, S., N. Aznamshah, and S. Jahanfar, A cross sectional study of microbial contamination of medical students' white coat. Malaysian Journal of Microbiology, 2007; 3(1): 35-38. https://doi.org/10.21161/mjm.00607
  22. Mohammadi, A., A. Ebrahimi, and S. Nemati, Bacterial and fungal contamination of elevator buttons in university schools of Isfahan University of Medical Sciences, Isfahan, Iran. Health Scope, 2016; 5(4). https://doi.org/10.17795/jhealthscope-49961
  23. Yarbrough, M.L., et al., Frequency of Instrument, Environment, and Laboratory Technologist Contamination during Routine Diagnostic Testing of Infectious Specimens. J Clin Microbiol, 2018; 56(6). https://doi.org/10.1128/JCM.00225-18
  24. Zazoli, M.A., et al., Investigation of microbial contamination of different wards in two teaching hospitals of Mazandaran University of Medical Sciences. Quarterly Journal of Health in the field, 2015; 1(3): 41-36. https://doi.org/10.5812/iji-130579
  25. Sewell, D.L., Laboratory-associated infections and biosafety. Clinical microbiology reviews, 1995; 8(3): 389-405. https://doi.org/10.1128/CMR.8.3.389
  26. Ghadjari, A., M. Asgari, and J. Burnie, Contamination of microscope slides with Aspergillus glaucus group A: a laboratory problem in the diagnosis of suspected mycotic infections. Journal of clinical pathology, 1997; 50(8): 699-700. https://doi.org/10.1136/jcp.50.8.699
  27. Neely, A.N. and M.M. Orloff, Survival of some medically important fungi on hospital fabrics and plastics. Journal of Clinical Microbiology, 2001; 39(9): 3360-3361. https://doi.org/10.1128/JCM.39.9.3360-3361.2001