بررسی آلودگی میکروبی در محیط و تجهیزات پزشکی بخش های مختلف دو بیمارستان آموزشی دانشگاه علوم پزشکی مشهد

نوع مقاله : Research Paper

نویسندگان

1 گروه آموزشی میکروب شناسی و ویروس شناسی دانشکده پزشکی

2 دانشجوی دکتری باکتری شناسی، گروه میکروب شناسی و ویروس شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

3 دانشجوی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

4 دپارتمان عفونی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

5 گروه پزشکی اجتماعی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد

چکیده

چکیده:
زمینه و هدف: عفونت‌های بیمارستانی همواره یکی از مشکلات عمده بهداشت و درمان می­باشد و روزبه‌روز بر اهمیت آن افزوده شده و چالشی برای نظام سلامت کشورها می­باشد. مطالعه حاضر با هدف بررسی میزان آلودگی در محیط و تجهیزات بیمارستان‌های قائم (عج) و امام رضا (ع) انجام شد.
مواد و روشها:این مطالعه مقطعی در دو بیمارستان دانشگاهی مشهد انجام شد. نمونه‌برداری به‌روش در دسترس از محیط و سطوح بخش‌های بالینی مختلف انجام گردید. تعداد و بازه نمونه‌برداری متغیر و بر اساس خط‌مشی کنترل عفونت هر بیمارستان در هر بخش تعیین شده بود. محل جمع‌آوری نمونه‌های محیطی شامل آب، سینک، تخت بیمار و انکوباتورها بود. نمونه­ها در محیط‌های کشت باکتری کشت داده شده و تعیین هویت گردید.
یافته‌ها: از 323 نمونه اخذ شده، 272 نمونه (2/84%) از بیمارستان قائم (عج) و 51 نمونه (8/15%) از بیمارستان امام رضا (ع) بود. 128مورد (1/47%) از نمونه‌های بیمارستان امام قائم و 17مورد (3/33%) از نمونه‌های بیمارستان امام رضا مثبت شدند (071/0=p). در مجموع بیشترین آلودگی مربوط به باسیلوس‌ها (7/16%)، استافیلوکوک اپیدرمیدیس (8/6%) و استافیلوکوک ساپروفیتیکوس (0/5%) بود. عوامل دیگر شامل استینوباکتر، انتروباکتر و سودوموناس بودند.
نتیجه‌گیری: گونه­های باسیلوس به‌دلیل اسپور ماندگاری بالایی در محیط دارند و حذف آنها شاخصی از بهداشت محیط مناسب خواهد بود. وجود باکتری‌های بالقوه بیماری‌زا در محیط بیمارستان، اهمیت برنامه­های کنترلی و نظارتی بهداشت محیطی را آشکار می­سازد.
نوع مقاله: پژوهشی

کلیدواژه‌ها


عنوان مقاله [English]

Investigation of microbial contamination in air and interior surfaces of two teaching hospitals of Mashhad University of Medical Sciences

نویسندگان [English]

  • masoud yosefi 1
  • Faria Hasanzadeh 2
  • Adeleh Tabatabeizadeh 3
  • Hamidreza Naderi 4
  • Majid Khadem Rezaiyan 5
1 medical university
2 PhD candidate of Microbiology, Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Medical Student, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4 Department of infectious diseases, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5 Department of Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده [English]

Abstract
Background and Aim: Nosocomial infections have always been one of the major health problems, and it is becoming increasingly important and a challenge for the health system of different countries. In this study, the contamination in the environment and equipment of two hospitals of Mashhad were studied.
Methods: This cross-sectional study was performed in two academic hospitals. The sampling method was convenient based on policy of infection control committee of each hospital. Environmental samples were collected from water, sink, patient bed and incubators. Samples were cultured in bacterial culture media and phenotypically identified. .
Results: Out of 323 samples, 272 (84.2%) were from Qaem Hospital and 51 (15.8%) from Imam Reza Hospital. Overall, 47.1% of samples of Imam Ghaem hospital (128 samples) and 33.3% of samples of Imam Reza hospital (17 samples) were positive (p = 0.071). Overall, the most prevalent organisms were Bacillus (54 samples, 16.7%) followed by Staphylococcus epidermidis (22 samples, 6.8%) and Staphylococcus saprophyticus (16 samples, 0.5%). Others were consisted of Acinetobacter, Enterobacter and pseudomonas spp.
Conclusion: Bacillus species have a high persistence in the environment due to Sporulation and their removal will be an indicator of proper environmental health. The presence of potentially pathogenic bacteria in the hospital environment reveals the importance of environmental health control and monitoring programs.
 

کلیدواژه‌ها [English]

  • Nosocomial infection
  • hospital contamination
  • Mashhad
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