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نوع مقاله : Research Paper

نویسندگان

1 دانشگاه علوم پزشکی مشهد - دانشکده بهداشت - گروه مهندسی بهداشت محیط و حرفه ای

2 دانشکده بهداشت

3 گروه مهندسی بهداشت محیط،دانشکده بهداشت،دانشگاه علوم پزشکی مشهد،مشهد،ایران

چکیده

زمینه و هدف: امروزه لامپ‌های فلوروسنت رایج‌ترین منبع نوری جهان هستند. میزان جیوه‌ موجود در این لامپ‌ها 115-72/0 میلی‌گرم به ازای هر لامپ بوده و با شکستن این لامپ‌ها، جیوه وارد محیط زیست شده و میزان سمیت آن، به شکل و راه ورود به بدن انسان بستگی دارد. جیوه در بدن تجمع یافته و باعث آسیب عصبی ، فلج و نابینایی می شود. یکی از بهترین روش‌ها برای کاهش جیوه، استفاده از میکروارگانیسم ها است. هدف از مطالعه حاضر، بررسی تأثیر باکتری‌های هتروتروف فاضلاب در حذف جیوه موجود در لامپ‌های فلوروسنت بود.
مواد و روش ها: در این مطالعه از لامپ‌های فلوروسنت خارج از رده استفاده شد. ابتدا توسط دستگاه خردایش ، اجزای مختلف لامپ‌ از هم جدا شدف و سپس جیوه آن‌ها با روش اسیدشویی از پودر فسفر لامپ جدا شد. باکتری‌های جدا شده از فاضلاب در معرض جیوه‌ی حاصل از اسیدشویی قرار گرفته و اثر این باکتری‌ها در کاهش جیوه مورد بررسی قرار گرفت.
یافته ها: نتایج مطالعه نشان داد ، باکتری‌های هتروتروف قادر به کاهش جیوه به کمتر از 5 میکروگرم بر لیتر می باشند و 19 باکتری هتروتروف جداسازی شده از فاضلاب نسبت به غلظت‌های 5 و 10 میلی‌گرم کلرید جیوه مقاومت نشان داده و بالاترین میزان کاهش جیوه،24/92 درصد، مربوط به باکتری سودوموناس مارجینالیس و کمترین میزان کاهش ،47/62%، مربوط به سودوموناس سیمیا بود.
نتیجه گیری: نتایح حاصل از این مطالعه نشان داد که باکتری‌های هتروتروف فاضلاب می‌توانند برای سم‌ زدایی جیوه از لامپ‌های فلوروسنت خارج از رده به عنوان یک روش کارآمد، کم هزینه و سازگار با محیط زیست استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of heterotrophic sewage bacteria of Mashhad on the removal of mercury from fluorescent lamp used in Mashhad

نویسندگان [English]

  • Hossein Alidadi 1
  • Fatemeh Mohammadhosseini 2
  • Samaneh Gohari 3
  • Ziaeddin Bonyadi 2

1 department of environmental health engineering ,mashhad university of medicine sciences

2 School of Health

3 Environmental Health Engineering Department, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده [English]

Background and Aim: Today, fluorescent lamps are the most common light source in the world and Iran. Mercury in these lamps may enter the environment and cause harmful effects. The aim of the present study was to investigate the effect of heterotrophic wastewater bacteria on the mercury removal from fluorescent lamps.
Materials and Methods: In this study, different components of lamps were separated using a crushing machine and then mercury was separated from phosphorus powder by acid washing. Bacteria isolated from wastewater were exposed to mercury from acid wash and the mercury content in the lamps was measured by atomic absorption spectrophotometer. The highest concentrations of mercury extracted from lamps was 86.03 ppb in the ratio of 4 HCL: 1 HNO3, and the lowest mercury concentration was14.03 ppb in the 1HCL: 1HNO3 ratio.
Results: The results of this study showed that heterotrophic bacteria can reduce mercury levels to less than 5 µg / L. 19 bacteria purified from wastewater were resistant to mercury chloride at concentrations of 5 and 10 mg. Also, 10 bacteria were able to reduce mercury. The highest rate of mercury reduction (92.24%) was related to Pseudomonas marginalis and the lowest rate (62.47%) was related to Pseudomonas simiae.
Conclusion: This study showed that heterotrophic sewage bacteria can be used as an efficient, low-cost, and environmentally friendly method for detoxification of mercury from out-of-date fluorescent lamps.

کلیدواژه‌ها [English]

  • Fluorescent Lamp
  • Mercury
  • Biosorption
  • Heterotrophic Wastewater Bacteria
1.         Subhavana K, Qureshi A, Roy A. Mercury levels in human hair in South India: baseline, artisanal goldsmiths and coal-fired power plants. Journal of exposure science & environmental epidemiology. 2019:1.
2.         Lecler M-T, Zimmermann F, Silvente E, Masson A, Morèle Y, Remy A, et al. Improving the work environment in the fluorescent lamp recycling sector by optimizing mercury elimination. Waste Management. 2018;76:250-60.
3.         Hobohm J, Krüger O, Basu S, Kuchta K, van Wasen S, Adam C. Recycling oriented comparison of mercury distribution in new and spent fluorescent lamps and their potential risk. Chemosphere. 2017;169:618-26.
4.         Kadam A, Nair GB, Dhoble S. Insights into the extraction of mercury from fluorescent lamps: A Review. Journal of Environmental Chemical Engineering. 2019.
5.         Al-Ghouti MA, Abuqaoud RH, Abu-Dieyeh MH. Detoxification of mercury pollutant leached from spent fluorescent lamps using bacterial strains. Waste management. 2016;49:238-44.
6.         Phanprasit W, Muadchim M, Park J, Robson MG, Sujirarat D, Kwonpongsagoon S, et al. Mercury health risk assessment among petrochemical workers in Rayong Province, Thailand. Human and Ecological Risk Assessment: An International Journal. 2019;25(6):1448-62.
7.         Abass K, Huusko A, Knutsen H, Nieminen P, Myllynen P, Meltzer H, et al. Quantitative estimation of mercury intake by toxicokinetic modelling based on total mercury levels in humans. Environment international. 2018;114:1-11.
8.         Liang P, Feng X, Zhang C, Zhang J, Cao Y, You Q, et al. Human exposure to mercury in a compact fluorescent lamp manufacturing area: By food (rice and fish) consumption and occupational exposure. Environmental pollution. 2015;198:126-32.
9.         Park J-H, Wang JJ, Xiao R, Pensky SM, Kongchum M, DeLaune RD, et al. Mercury adsorption in the Mississippi River deltaic plain freshwater marsh soil of Louisiana Gulf coastal wetlands. Chemosphere. 2018;195:455-62.
10.       Liu L, Bilal M, Duan X, Iqbal HM. Mitigation of environmental pollution by genetically engineered bacteria—Current challenges and future perspectives. Science of The Total Environment. 2019.
11.       Bonsignore M, Andolfi N, Barra M, Madeddu A, Tisano F, Ingallinella V, et al. Assessment of mercury exposure in human populations: A status report from Augusta Bay (southern Italy). Environmental research. 2016;150:592-9.
12.       Ozgur C, Coskun S, Akcil A, Beyhan M, Üncü IS, Civelekoglu G. Combined oxidative leaching and electrowinning process for mercury recovery from spent fluorescent lamps. Waste management. 2016;57:215-9.
13.       Wang J, Hong Y, Lin Z, Zhu C, Da J, Chen G, et al. A novel biological sulfur reduction process for mercury-contaminated wastewater treatment. Water research. 2019;160:288-95.
14.       Imron MF, Kurniawan SB, Soegianto A. Characterization of mercury-reducing potential bacteria isolated from Keputih non-active sanitary landfill leachate, Surabaya, Indonesia under different saline conditions. Journal of environmental management. 2019;241:113-22.
15.       Huang Z, Wei Z, Xiao X, Tang M, Li B, Zhang X. Simultaneous mercury oxidation and NO reduction in a membrane biofilm reactor. Science of The Total Environment. 2019;658:1465-74.
16.       Giovanella P, Cabral L, Bento FM, Gianello C, Camargo FAO. Mercury (II) removal by resistant bacterial isolates and mercuric (II) reductase activity in a new strain of Pseudomonas sp. B50A. New biotechnology. 2016;33(1):216-23.
17.       Su Y-Q, Zhao Y-J, Zhang W-J, Chen G-C, Qin H, Qiao D-R, et al. Removal of mercury (II), lead (II) and cadmium (II) from aqueous solutions using Rhodobacter sphaeroides SC01. Chemosphere. 2019:125166.
18.       Nabavi BF, Nikaeen M, Amin MM, Hatamzadeh M. Isolation and identification of aerobic polychlorinated biphenyls degrading bacteria. International Journal of Environmental Health Engineering. 2013;2(1):47.
19.       Jang M, Hong SM, Park JK. Characterization and recovery of mercury from spent fluorescent lamps. Waste management. 2005;25(1):5-14.
20.       Rhee S-W, Choi H-H, Park H-S. Characteristics of mercury emission from linear type of spent fluorescent lamp. Waste management. 2014;34(6):1066-71.
21.       Rey-Raap N, Gallardo A. Determination of mercury distribution inside spent compact fluorescent lamps by atomic absorption spectrometry. Waste Management. 2012;32(5):944-8.
22.       Park H-S, Rhee S-W. Estimation of retorted phosphor powder from spent fluorescent lamps by thermal process. Waste management. 2016;50:257-63.
23.       Hobohm J, Kuchta K, Krüger O, van Wasen S, Adam C. Optimized elemental analysis of fluorescence lamp shredder waste. Talanta. 2016;147:615-20.
24.       Innocenzi V, Ippolito NM, De Michelis I, Medici F, Vegliò F. A hydrometallurgical process for the recovery of terbium from fluorescent lamps: experimental design, optimization of acid leaching process and process analysis. Journal of environmental management. 2016;184:552-9.
25.       Kafilzadeh Farshid MN, Kargar Mehdi. Isolation and identification of mercury-resistant bacteria from water and sediments of the Kerr River. Journal of the World of Microbes.1(1):43-9 [In Persian].
26.       Aram M, Sharifi A, Kafeelzadeh F, Naghmachi M, Yasari E. Isolating mercury-resistant bacteria from Lake Maharloo. International Journal of Biology. 2012;4(3):63.
27.       Kargar M, Jahromi MZ, Najafian M, Khajeaian P, Nahavandi R, Jahromi SR, et al. Identification and molecular analysis of mercury resistant bacteria in Kor River, Iran. African Journal of Biotechnology. 2012;11(25):6710-7.
28.       Dash HR, Mangwani N, Das S. Characterization and potential application in mercury bioremediation of highly mercury-resistant marine bacterium Bacillus thuringiensis PW-05. Environmental Science and Pollution Research. 2014;21(4):2642-53.
29.       Dash HR, Das S. Bioremediation of inorganic mercury through volatilization and biosorption by transgenic Bacillus cereus BW-03 (pPW-05). International Biodeterioration & Biodegradation. 2015;103:179-85.