Journal of Research in Environmental Health

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

News

Editorial Policy

Advertising Policy

Prevalence of Antibiotic-Resistant Strains of Yersinia Enterocolitica Isolated from Traditional and Industrial Dairy Products Sold in Isfahan County

Document Type : Research article

Authors

1 Graduated in Food Hygiene, Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

2 Professor, Department of Food Hygiene, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Abstract

Background and purpose: Milk and its products are a suitable environment for the growth of pathogenic microorganisms, among the cold-loving pathogens Yersinia enterocolitica is one of the bacteria whose spread can threaten the health of consumers. The aim of the present study was to Prevalence of antibiotic-resistant strains of Yersinia enterocolitica isolated from traditional and industrial dairy products sold in Isfahan county.

Materials and Methods: First, 300 samples of traditional and industrial dairy products were sampled and transferred to the laboratory, and the level of contamination with Y. enterocolitica was determined by standard methods. Multiplex PCR method was used to determine the frequency of virulence genes and Disk-Diffusion method was used to evaluate antibiotic resistance.

Results: The results showed that the level of contamination with Yersinia enterocolitica was 15 samples (7.5%) out of a total of 200 traditional dairy samples, and none of the industrial samples were contaminated with Yersinia enterocolitica. According to the PCR test, the frequency of the highest and lowest carrier genes, respectively, includes ail 14 samples (4.66‌%), yadA 7 samples (2.33 %), inv 10 samples (3.33 %), yst 9 samples (3 %), rfbe 9 samples (3%) and virF 6 samples (2%). The results of antibiotic resistance showed that the highest resistance was related to penicillin (80%) and ampicillin (73.33%), and the lowest resistance was to imipenem (6.66%).

Conclusion: The results of this study showed that traditional dairy products were contaminated with Yersinia enterocolitica bacteria and had virulence genes, so the use of traditional dairy products should be limited and the use of antibiotics should be limited in the event of illness.
 
Open Access Policy: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/

Keywords

References
  1. Rana, T. The Microbiology, Pathogenesis and Zoonosis of Milk Borne Diseases: Milk Hygiene in Veterinary and Public Health. Elsevier 2024. P. 51-68.‏.
  2. Bhattacharya, D., Das, A., Sarkhel, S. P., Satyaprakash, K. Milk-borne parasitic zoonoses. In The Microbiology, Pathogenesis and Zoonosis of Milk Borne Diseases. 2th ed. Academic Press. 2024. P. 295-304. ‏ https://doi.org/10.1016/B978-0-443-13805-8.00016-8
  3. Owusu-Kwarteng, J., Akabanda, F., Agyei, D., Jespersen, L. Microbial safety of milk production and fermented dairy products in Africa. Microorganisms. 2020. 8(5):740- 752. https://doi.org/10.3390/microorganisms8050752 PMid:32429521 PMCid:PMC7285323
  4. Banach, J., Van, J., Kleter, A. Alternative proteins for meat and dairy replacers: Food safety and future trends. Cri Rev Food Sci Nut. 2023. 63(32):11063-11080. https://doi.org/10.1080/10408398.2022.2089625 PMid:35757863
  5. Sharifi Yazdi, S., Mobasseri ,B., Torabi Bonab, P., Mirbagheri, Z., SoltanDallal, MM., Prevalence and Characteristics of Yersinia Enterocolitica and Yersinia Pseudotuberculosis from Raw Milk Supplied in Tehran. J Nut Food Sec. 2023. 8(1):114-21. https://doi.org/10.18502/jnfs.v8i1.11776
  6. Platt-Samoraj, A. Toxigenic properties of Yersinia enterocolitica biotype 1A. Toxins. 2022. 14(2):118-127. https://doi.org/10.3390/toxins14020118 PMid:35202145 PMCid:PMC8877543
  7. Hurst, MR., Beattie, AK., Jones, SA., Hsu, P-C., Calder, J., van Koten, C., Temperature-dependent Galleria mellonella mortality as a result of Yersinia entomophaga infection. App Env Mic. 2015. 81(18): 6404-6414. https://doi.org/10.1128/AEM.00790-15 PMid:26162867 PMCid:PMC4542262
  8. Riahi, SM., Ahmadi, E., Zeinali, T. Global prevalence of Yersinia enterocolitica in cases of gastroenteritis: A systematic review and meta‐analysis. Int J mic. 2021. 25(1): 1-17. https://doi.org/10.1155/2021/1499869 PMid:34512763 PMCid:PMC8433020
  9. Lü, Z., Su, X., Chen, J., Qin, M. Prevalence, bio-serotype, antibiotic susceptibility and genotype of Yersinia enterocolitica and other Yersinia species isolated from retail and processed meats in Shaanxi Province, China. LWT. 2022. 168(1):113-122. https://doi.org/10.1016/j.lwt.2022.113962
  10. Fois, F., Piras, F., Torpdahl, M., Mazza, R. Prevalence, bioserotyping and antibiotic resistance of pathogenic Yersinia enterocolitica detected in pigs at slaughter in Sardinia. Int J food mic. 2018. 283(1):1-6. https://doi.org/10.1016/j.ijfoodmicro.2018.06.010 PMid:29929063
  11. Ahmed, A., Diab, H., Hendy, B., Batiha, G. Molecular Characterization of Y. enterocolitica Isolated from Dairy Environment with Special Reference to the Antimicrobial Activity of Milk Proteins Hydrolysates. J Adv Vet Res. 2022. 12(2):118-127.
  12. Khalid, D., Abbas, B. Prevalence, antibiotic susceptibility, and virulence factors of Yersinia enterocolitica isolated from raw milk in Basrah, Iraq. Bulg J Vet Med. 2021. 24(1):17-23. https://doi.org/10.15547/bjvm.2019-0044
  13. Salimi, F., Bonyadian, M., Moshtaghi, H., Virulence Genes and Antibiotic Resistance Pattern of Yersinia Entrocolitica Isolated from Raw Milk and Traditional Cheeses. J Mic Biol. 2024. 12(47):63-76.
  14. Rahimi, E., Sepehri, S., Dehkordi, FS., Shaygan S., Momtaz, H. Prevalence of Yersinia species in traditional and commercial dairy products in Isfahan Province, Iran. Jundi J Mic. 2014. 7(3):75-86. https://doi.org/10.5812/jjm.9249
  15. Darwish, SF., Asfour, HA., Allam, HA. Incidence of Yersinia enterocolitica and Yersinia pseudotuberculosis in Raw Milk Samples of Different Animal Species Using Conventional and Molecular Methods. Alex J Vet Sci. 2015. 44(1):12-17. https://doi.org/10.5455/ajvs.176360
  16. Saberianpour, S., Tajbakhsh, E., Khamesipour, F., Branch, S. Prevalence of virulence genes and biotyping of Yersinia enterocolitica isolated from chicken meat in Shahrekord, Iran. Int Res J. 2014. 3(2):71-6.
  17. Heidarzadi, M., Rahnama, M., Alipoureskandani, M., Saadati ,D. Salmonella and Escherichia coli contamination in samosas presented in sistan and baluchestan province and antibiotic resistance of isolates. 2021.42(2):81-92.
  18. Özdemir, F., Arslan, S. Genotypic and phenotypic virulence characteristics and antimicrobial resistance of Yersinia spp. isolated from meat and milk products. J food sci. 2015. 80(6): 1306-1313. https://doi.org/10.1111/1750-3841.12911 PMid:25969137
  19. Piras, F., Siddi, G., Le Guern, A-S. Traceability, virulence and antimicrobial resistance of Yersinia enterocolitica in two industrial cheese-making plants. Int J Food Mic. 2023. 39(8):110-125. https://doi.org/10.2139/ssrn.4359811
  20. Dowidar, HA., Khalifa, MI. Virulence Genotyping Profiles of Cefazolin Resistance Yersinia enterocolitica Isolated From Milk and Milk Products in Egypt. Egyp J Vet Sci. 2023. 54(4):555-569. https://doi.org/10.21608/ejvs.2023.197879.1455
  21. Piras, F., Spanu, C., Sanna, R. Detection, virulence genes and antimicrobial resistance of Yersinia enterocolitica in sheep and goat raw milk. Int Dairy J. 2021.117(1):105-111. https://doi.org/10.1016/j.idairyj.2021.105011
  22. Khalid, D., Abbas, B. Prevalence, antibiotic susceptibility, and virulence factors of Yersinia enterocolitica isolated from raw milk in Basrah, Iraq. Bulg J Vet Medi.2024. 21(1): 17-23.
  23. Abdelwahab, AM., El-Tawab, A. Phenotypic and genotypic studies on antibiotic resistant Yersinia enterocolitica isolated from milk and milk products in Kaliobia, Egypt. Ben Vet Med J. 2021;40(2): 149-153. https://doi.org/10.21608/bvmj.2021.66845.1358
  24. Alavi, SM., Rahimi, E., Tajbakhsh, E, Identification and characterization of the virulence genes in Yersinia enterocolitica strains isolated from sheep and goat milk in Shahrekord. J Mic World. 2017.10(3): 256-262.
  25. Movafagh, F., Zeinali, T., Jamshidi, A. Contamination Rate of Bovine Raw Milk with Yersinia enterocolitica Biotypes in Mashhad, Iran. J Env Health Pro. 2021.7(1):30-34. https://doi.org/10.52547/jhehp.7.1.30
  26. Ahmed, HA., Tahoun, AB. Prevalence of Yersinia enterocolitica in milk and dairy products and the effects of storage temperatures on survival and virulence gene expression. Int dairy J. 2019. 94(1): 16-21. https://doi.org/10.1016/j.idairyj.2019.02.010
  27. Tavassoli, M., Jamshidi, A., Ranjbar, G. Prevalence, biotyping, and antimicrobial resistance of Yersinia enterocolitica isolated from traditional Iranian cheeses-evaluation of yersinia enterocolitica in traditional cheeses. Food hyg. 2021. 12(7):130-139. https://doi.org/10.15835/buasvmcn-fst:2020.0051
  28. Bonardi, S., Leguern, A. Detection, virulence and antimicrobial resistance of Yersinia enterocolitica in bulk tank milk in Italy. Int dairy J. 2018;84(1):46-53. https://doi.org/10.1016/j.idairyj.2018.04.003
  29. Ye, Y., Ling, N, Han, Q. Detection and prevalence of pathogenic Yersinia enterocolitica in refrigerated and frozen dairy products by duplex PCR and dot hybridization targeting the virF and ail genes. J dairy sci. 2014. 97(11):6785-6791. https://doi.org/10.3168/jds.2014-8382 PMid:25218752
Journal of Research in Environmental Health
Volume 10, Issue 3 - Serial Number 39
December 2024
Pages 99-111
Files
Share
How to cite
Statistics