Document Type : Research article
Authors
- Ali Toolabi 1
- Naser Torbati Zare 2
- Yasan Kazem Zade 3
- Hamid Sarhadi 4
- Abozar Raisvand 5
- Ziaeddin Bonyadi 6
1 1Assistant Professor, Environmental Health Engineering Department, Faculty of Health, Bam University of Medical Sciences, Bam, Iran
2 2 MSc, Food Engineering and Science, Biotechnology, Bam University of Medical Sciences, Bam, Iran
3 MSc, Epidemiology Department, Khomein University of Medical Sciences, Khomein, Iran
4 Islamic Azad University of Bam
5 Master of Epidemiology, Vice Chancellor for Health, Qazvin University of Medical Sciences, Qazvin, Iran
6 Assistant Professor, Environmental Health Engineering Department, Faculty of Health, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract
Background and purpose: Today, due to the dangers of chemical preservatives in food, alternative and safe methods such as the use of natural extracts of natural plants have increased. The aim of this study was to evaluate the antibacterial effects of Kenar fruit extract on the bacteria of Salmonella, Shigella, Escherichia coli and Bacillus cereus.
Materials and Methods: We prepared Kenar fruit extract by soaking or immersing in methanol. After concentrating by rotary device, we prepared 9 concentrations of the extract serially, then performed antibiogram by well method and determined MIC and MBC. We also used volumetric-volumetric solution of methanol and dimethyl sulfoxide in equal proportions as negative control and control disks and standard commercial antibiotics as positive control.
Results: Based on the results, the diameter of inhition zone for dropletsfor Shigella, Salmonella, Bacillus cereus and Escherichia coli at a concentration of 80 mg/mL of methanolic extract of the plant was 24, 17, 59 and 19 mm, respectively. It was also found that methanolic extract of Kenar fruit had very good antibacterial effects on gram-positive bacterium Bacillus cereus compared to other target bacteria (which are gram-negative) and had significant growth inhibition and bactericidal effects at very low concentrations.
Conclusion: The results showed that methanolic extract had very favorable antibacterial effects against four target bacteria. Therefore, it can be said that the compounds of methanolic extract of this plant can be used as important components of various products in the food, pharmaceutical, cosmetic and health industries.
Keywords
- Russell, N. J. and G.W. Gould (2003). Food preservatives. 2th ed New York: Springer Science & Business Medi; p.150-161.
- Prakash, B., A. Kedia, P. K. Mishra and N. Dubey (2015). "Plant essential oils as food preservatives to control moulds, mycotoxin contamination and oxidative deterioration of agri-food commodities Potentials and challenges." Food Control.2015; 47: 381-391.
- Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World Agricultur Sci 2008; 4:839-43.
- Sagdic O, Karahan AG, Ozcan M, Ozcan G. Note effect of some spices extracts on bacterial inhibition. Food Sci Technol Int 2003; 9:353-9
- Motamedi H, Safary A, Maleki S, Seyyednejad S M. Ziziphusspina-christi, a native plant from khuzestan, iran, as a poteneial source for discovery new antibacterial agents. Asian J Plant Sci 2009; 8: 187-90.
- Choi SH, Ahn JB, Kim HJ, Im NK, Kozukue N, Levin CE, et al. Changes in free amino acid, protein, and flavonoid content in jujube (Ziziphus jujube) fruit during eight stages of growth and antioxidative and cancer cell inhibitory effects by extracts. J Agric Food Chem. 2012; 60:10245–55
- Okogun JI (2000). Methods of Medicinal Plant Extract Preparation. National Institute for Pharmaceutical Research and Development (NIIPRD). Idu – Abuja, Nigeria.
- Shahabi Rabari Alfr, Khairkhah B. Antibacterial effects of peppermint, cockatiel and lemon balm Kermani. First edition. Iran Cup Publications. 1397.
- Odland BA, Erwin ME, Jones RN (2000): Quality control guidelines for disk diffusion and broth microdilution anti- microbial susceptibility tests with seven drugs for veterinar y applications. Journal of Clinical Microbiology 38, 453–455.9.
- Murray PR. Baron EJ. Jorgensen JH. LandryML. Pfaller MA. 2003. Listeria and ErysipelothrixMan Clin Microbiol; 1: 461 9.
- Odland BA, Erwin ME, Jones RN (2000): Quality control guidelines for disk diffusion and broth microdilution anti- microbial susceptibility tests with seven drugs for veterinar y applications. Journal of Clinical Microbiology 38, 453–455.
- Jay, J.M., Modern food microbiology. 2010. Aspen puplication Gaithersburg. Mary land., pp: 120 - 3.
- Rezaei-Zarchi S, Javed A, Javeed Ghani M, Mirjalili H, Moghaddam B. Pathogenic strain of Escherichia coli. Ir. J of Pathology. 2010; 5:83-89.
- Arjang M. Dakhili m, Farahani F. 1394. Investigation of chemical compounds and antimicrobial activity of Lemongrass extract. Journal of Qom University of Medical Sciences, 9, 2015, 7-13
- Kraft K, Hobbs C. Pocket Guide to Herbal Medicine. New York: Thieme Stuttgart; 2004. p. 61-62.
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