Narjes Bagheri; Giti Kashi; Hamid Reza Tashauoei
Abstract
AbstractBackground and Aim: Helicobacter pylori is the most common gastrointestinal pathogen infecting more than half of the world's population. Helicobacter pylori is a bacterium that causes gastrointestinal ulcers (chronic gastritis), stomach cancer, lymphoma, and adenocarcinoma. This study aimed to ...
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AbstractBackground and Aim: Helicobacter pylori is the most common gastrointestinal pathogen infecting more than half of the world's population. Helicobacter pylori is a bacterium that causes gastrointestinal ulcers (chronic gastritis), stomach cancer, lymphoma, and adenocarcinoma. This study aimed to apply the Polymerase Chain Reaction (PCR) for investigating Helicobacter pylori in the tap water of selected hospitals in Tehran in 2020.Materials and Methods: In this study, 22 tap water samples and six well water samples were randomly gathered from the selected hospitals in different areas of Tehran from September 5 to November 20, 2020. The samples were collected in sterile bottles according to the procedure detailed in national standard methods. In this study, Helicobacter pylori was evaluated using Polymerase Chain Reaction (PCR) and surface culture. Data analysis was done by SPSS18 software. Results: This study showed Helicobacter pylori in two samples (10.3%) by PCR. The mean values of Helicobacter pylori in tap water and well water samples were 0.18 ± 0.85 and 0.67±1.63, respectively. The mean values of heterotrophic plate count in tap water and well water samples were 0.00 ±0.00 and 7.83±2.10, respectively. The mean values of coliforms, Escherichia coli, Pseudomonas aeruginosa, Clostridium perfringens, and Streptococcus faecalis in tap water were 0.00 ±0.00.Conclusion: Helicobacter pylori has high persistence in the aquatic environment due to resistance in harsh environments and its absence will be an indicator of proper environmental health. So, according to our results, infection control and preventive strategies to reduce the risk of exposure to Helicobacter pylori for safe water supply are purposed to public health authorities. The evaluation of the biological quality of water (heterotrophic bacteria, coliforms, Escherichia coli, Pseudomonas aeruginosa, Clostridium perfringens, and Streptococcus faecalis), physicochemical quality of water, and Helicobacter pylori in water is among the strengths and innovations of this research.
A. shahryari; N. Ahmadi; Y. Dadban Shahamat; R. Aali; A. Mokarary; J. Sharifi Ghojagh
Abstract
Background & objective: There is a public concern about the microbial quality of distribution water systems that it induces increasingly consumption of bottled waters in our country. In Iran, bottled waters are produced in two packaging models; small (0.5 Litter) and big (1.5 Litter); microbial load ...
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Background & objective: There is a public concern about the microbial quality of distribution water systems that it induces increasingly consumption of bottled waters in our country. In Iran, bottled waters are produced in two packaging models; small (0.5 Litter) and big (1.5 Litter); microbial load of which can be deteriorated before using by consumer and durrin storage. This study was design to assess the bacterial contamination of bottled waters and compare the ratio of contamination in small and big bottles packaged. Materials & Methods: in this descriptive and analytic study, 400 bottled water samples including 185 of small and 215 of big bottled waters were selected from Gorgan food markets during May 2014 to April 2015 from 10 popular brands. Microbial level of bottled waters were determined via Heterotrophic plate count (HPC) on the R2A medium with spread plated method. For statistical analyses, SPSS ver.23.0 were used by one sample T test and chi-square test in a P-value ofResults: The mean HPC bacteria were 8557 and 11188 Colony Forming Unit per milliliter (CFU/ml) in small and big bottled waters, respectively, which are much higher than microbial standard (500 cfu/ml) set by drinking water. Moreover, in 85.0 percent of all samples tested, HPC level was more than 500 cfu/ml; that 40.8 and 44.2 percent of its related to small and big bottles, respectively. The statistical analysis showed that the quantity of bottles water did not have a significant effect on the number of bacteria. Conclusion: Small and big bottled waters had a microbial contamination based on the heterotrophic plate count. Therefore, consumption of bottled water could be hazardous for public health particularly for lowered immunity.