زیست افزایی خاک های آلوده به کروزن، به‌وسیله سودوموناس پوتیدا و سراشیا مارسسنس جداشده از لجن فعال و بررسی میزان رشد آنها

نوع مقاله: Research Paper

نویسندگان

1 - دانشیار، دانشگاه آزاد اسلامی، واحد جهرم، گروه زیست‌شناسی، جهرم، ایران

2 کارشناسی ارشد ، دانشگاه آزاد اسلامی، واحد جهرم، گروه زیست‌شناسی، جهرم، ایران.

چکیده

زمینه و هدف:  زیست‌افزایی، یکی از روش‌های برتر در اصلاح زیستی خاک‌های آلوده به هیدروکربن‌های نفتی است. هدف از این پژوهش، ارزیابی میزان تأثیر باکتری‌های جداشده از لجن فعال به‌عنوان باکتری‌های غیربومی، در اصلاح زیستی خاک‌های آلوده به کروزن و بررسی رشد باکتری‌های جداشده در حضور غلظت‌های متفاوت این فرآورده است.
مواد و روش‌ها:  نمونه‌برداری از لجن فعال دو کمپ تصفیه‌خانه منطقه عسلویه صورت گرفت. جداسازی باکتری‌های تجزیه‌کننده با کشت نمونه‌ها روی محیط پایه معدنی انجام شد. تست امولسیفیکاسیون و ارزیابی رشد باکتری‌ها در غلظت‌های متفاوت کروزن صورت گرفت. برای زیست فزونی و سنجش میزان پاک‌سازی زیستی، باکتری‌های جداشده به ترکیب نفتی کروزن به خاک‌های آلوده تلقیح شدند و میزان تجزیه زیستی به‌وسیله دستگاه طیف‌سنجی مادون‌قرمز (IR) اندازه‌گیری شد.
یافته‌ ها:  در این مطالعه، 2 باکتری سودوموناس پوتیدا و سراشیا مارسسنس از لجن فعال، به‌عنوان باکتری‌های تجزیه‌کننده کروزن جداسازی و شناسایی شدند. با توجه به تست‌های امولسیفیکاسیون، سنجش رشد باکتری‌ها در غلظت‌های گوناگون کروزن و نتایج به‌دست‌آمده از زیست‌افزایی ستون‌های خاک‌آلوده به کروزن و کاهش سطح TPHs، باکتری سودوموناس پوتیدا به‌عنوان قوی‌ترین باکتری تجزیه‌کننده این فرآورده نفتی شناخته شد.
 نتیجه‌گیری:  با توجه به سازگار شدن باکتری‌های لجن فعال با انواع آلاینده‌های موجود در فاضلاب، می‌توان از آ‌نها به‌عنوان باکتری‌های غیربومی برای زیست‌افزایی و پاک‌سازی خاک‌های آلوده به هیدروکربن‌های نفتی استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Bioaugmentation of kerosene from contaminated soils by Pseudomonas putida and Serratia marcescens isolated of the activated sludge and a study on their growth

نویسندگان [English]

  • Farshid Kafilzadeh 1
  • Zeinab Khaledi 2
1 Associated professor, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
2 Msc, Department of Biology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
چکیده [English]

Background & Objective: Bioaugmentation is a great technique in bioremediation of contaminated soils with petroleum hydrocarbons. The aim of this study was to evaluate the effect of isolated bacteria from activated sludge as non-indigenous bacteria in bioremediation of kerosene contaminated soils and study the growth of isolated bacteria in the presence of different concentrations of this product.

Materials & Methods: Sampling of activated sludge was taken from two treatment plants in Assaluyeh region. Isolation of degrading bacteria was performed by culturing the samples on basal mineral medium. Emulsification test and evaluating growth of bacteria were carried out in different concentrations of kerosene. Isolated bacteria were inoculated to polluted soils with kerosene oil compound for bioaugmentation. The evaluation of their bioremediationpotentialand the rate of biodegradation were measured by infrared spectroscopy (IR).

Results: In this study, two bacteria Pseudomonas putida and Serratia marcescens were isolated and identified as kerosene degrading bacteria from activated sludge. P. putida was recognized as the most powerful degrading bacterium of this oil product according to emulsification tests, measuring the growth of bacteria in various concentrations of kerosene, the results of Bioaugmentation of contaminated column of soil with kerosene and reduce the level of TPHs.
   
Conclusion: In regard to adaptation of activated sludge bacteria with variety of pollutants in sewage, they can be used as non-indigenous bacteria for bioaugmentation and cleaning up contaminated soil with petroleum hydrocarbons.

کلیدواژه‌ها [English]

  • Bioaugmentation
  • Kerosene
  • Activated sludge
  • Pseudomonas putida
  • Serratia marcescens
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