بررسی تاثیر غلظت های مختلف شوری بر روی رشد میکروجلبک کلرلا (Chlorella sp.) جمع آوری شده از شالیزارهای استان گلستان

نوع مقاله: مقالات پژوهشی

نویسندگان

1 استادیار

2 مربی، مدرس گروه محیط زیست، موسسه آموزش عالی بهاران، گرگان، ایران

3 دانشجوی کارشناسی ارشد مهندسی منابع طبیعی- محیط زیست (آلودگی محیط زیست)، موسسه آموزش عالی بهاران، گرگان، ایران

چکیده

چکیده
زمینه و هدف: شوری، یکی از مهم‌ترین عوامل مؤثر در رشد و تولید متابولیت­های ارزشمند در کشت انبوه جلبک‌ها می­باشد. مطالعه حاضر با هدف بررسی تأثیر شوری بر رشد و وضعیت رنگیزه‌ای جلبک کلرلا. جمع‌آوری شده از شالیزارهای استان گلستان جهت ارزیابی توانمندی ورود به کشت انبوه انجام شد.
مواد و روش‌ها:  به منظور تأثیر شوری بر رشد و وضعیت رنگیزه‌ای جلبک کلرلا ، تخلیص در محیط کشت N8 انجام شد و نمونه خالص تحت شدت نور 2 میکرومول کوانتا بر متر مربع در ثانیه در دمای 28 درجه سانتی‌گراد و روشنایی مستمر فلورسنت و pH 7/2قرار گرفت. تیمار شوری اعمال شده از نوع کلرور سدیم به میزان 0%، 0/25%، 0/5% و 1% ( 17 تا 170 میلی‌مولار) بود.
یافته‌ها: از روز پنجم پس از تلقیح، رشد صعودی در تیمار و شاهد مشاهده شد. رشد بهینه و بالاترین نرخ رشد ویژه در شوری 0/5% مشاهده گردید. رشد در شرایط شاهد و شوری در حد 1% و 0/5% معنی‌دار بود. میزان تولید رنگیزه کاروتنوئید در روز نهم پس از تلقیح در شوری 1% از بقیه تیمارها بیشتر بود و در میزان محتوای کلروفیل تغییر معناداری مشاهده نشد.
نتیجه‌گیری:جلبک کلرلا قابلیت خوگیری با غلظت شوری­های مورد بررسی در این پژوهش را دارا می­باشد و از این نظر می­توان آن را از لحاظ تلقیح در زمین­های کشاورزی به عنوان کود زیستی و اصلاح‌گر خاک در استان گلستان مورد توجه قرار داد.
نوع مقاله: مقاله پژوهشی

کلیدواژه‌ها


عنوان مقاله [English]

Studying of different salinity concentrations on the growth microalgae "chlorella sp" from rice fields Golestan Province

نویسندگان [English]

  • Mahdi Sadeghi 1
  • Sarah Jorjani 2
  • Ali Shahbazi 2
  • Kazem Babaei ziyarati 3
1 Assistant professor of Environmental Health Research Center,Golstan University of Medical Sciences, Gorgan, Iran
2 MSc of Environmental Engineering ,Department of Environmental Engineering, Baharan Higher Education Institute, Gorgan, Iran
3 MSc student of Natural Resources Engineering-Environmental Science-Environmental Pollution,Department of Environmental Engineering, Baharan Higher Education Institute, Gorgan, Iran
چکیده [English]

Background and Aim: Salinity is one of the most important factors affecting growth, photosynthesis and valuable metabolites regarding large scale cultivations. The aim of this research was studying the effect of salinity on growth and pigment composition of Chlorella sp. Collected from paddy-fields of the Golestan Province for evaluating of the potentiality for large scale cultivation.
Materials and Methods: Purification was done using N8 culture media at 2 uE.m-2.S-1 irradiance, 280C and continuous illumination at pH 7.2. Salinity treatments were Sodium Chloride at 0, 0.25.0.5 and 1% ( 17 to 170 mM) concentration. Survival and growth were assayed by turbidity, pigment composition as in vivo and in vitro were measured after normalization at the dry weight unit.
Results: Results showed that the sharp increase in growth rate viewed at 5th day after inoculation at both control and treatments. Survivality would be keep at this salinity range. The optimum of growth and the highest degree of special growth rate were seen in 0.5% salinity. Difference between the growth at 0.5 and 1 % salinity were significant. The ability of carotenoide production at the 9th day after inoculation were significantly more than the other treatments and insignificant for chlorophyll production.
Conclusion: The results show that Chlorella sp., have acclimatized potentiality against this degree of tension and regarding that would be considered as a suitable candidates for future bio fertilizer and soil conditioner inoculation in Golestan province.
Document Type: Research ar

کلیدواژه‌ها [English]

  • "Microalgae"
  • "Chlorella"
  • "Acclimatization"
  • "Salinity"
  • 'Bio fertilize"

 

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